FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3459907
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC145740.42122519478124704No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC122500.3540557593022009No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA122020.35266843877595555No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG99870.28864937699192494No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT69340.2004100110205274No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC40820.11798004975278238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG19850.061.23925
GTCTACG20100.060.6498224
TACGGCC20350.059.9047437
CTACGGC21450.056.994166
GCGCGGT71250.054.09959
ACGGCCA23750.051.6205448
GCCATAC23600.051.508411
CGGCCAT23950.050.9002659
TGGCGCG76000.050.71827715
CCGAACT11350.050.69827767
CGCGGTG78050.049.29741310
CGGTTCA11950.048.9780775
GGGTCTA19900.048.3809782
GCCGGTT12350.047.672173
CCATACC25500.047.67051712
CATACCA26200.046.52906413
GGCCATA26450.046.48208610
CGAACTT12600.046.3984268
CCTGGTT37450.045.39457370
GAACGCG27450.043.9418124
Produced by FastQC (version 0.11.5)