FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458177
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG189540.5480922462904588No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC102180.2954735977944449No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC84600.2446375648210025No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG73720.21317590163834874No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG72020.20826001676605913No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA56840.16436405655349623No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT49280.14250282735672581No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG47060.13608326005291227No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA45760.13232405397410255No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT41330.11951383633631246No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT40550.11725831268902662No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG36250.10482401565911752No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG35630.10303116352922362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA53100.037.7759232
CCCGTCG51650.036.97847444
TCGCCGG53050.036.18002348
GCGGCAC55450.036.1181333
CGGCACC56500.035.39214734
CCGTCGC54150.035.21518745
GTTCCGT3200.034.9238781
TGGCCGT12400.033.96713370
GCACCCC60350.033.37603436
CCCCCGA61850.032.60275319
ACCCGCG62000.032.48820525
CACCCCC62550.032.0962237
GGCACCC66150.030.49506435
CCCCGAC67300.029.91324220
GTCCGTT2550.029.8927612
ATGGCCG11700.029.0916169
GATAGGC30650.028.23330340
GTGCCGT5600.027.4401911
TCAGGAT19650.026.98254270
GCATTAG32350.026.87632612