FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458177
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC139690.4039411516530241No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA118390.3423480059002185No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC117150.33876230164043075No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG94880.2743642098134364No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT68940.19935359005626374No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC39910.11540762661945873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC20000.059.0496567
GTCTACG19950.059.0206384
TCTACGG20450.057.411565
CTACGGC20850.056.3101436
GCGCGGT66950.053.850149
CCGAACT11550.052.56853567
CGAACTT11750.052.4850668
ACGGCCA22850.051.533038
CGGTTCA11850.051.438385
GCCATAC22850.051.38072211
GCCGGTT11950.051.2947853
TGGCGCG71200.050.6829515
CGCGGTG73500.049.19260810
CGGCCAT24250.049.128499
GGGTCTA19300.047.9303252
CCGGTTC12950.047.8686454
CCATACC24950.047.4711412
GGCCATA26000.045.82176210
CGGATTG3600.045.5790331
GCGTCTA5050.045.280272
Produced by FastQC (version 0.11.5)