FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726455
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG193050.5180526800940841No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN101600.27264518154653683No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG56950.1528262115066464No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG49780.13358540489553744No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA45680.12258299107328546No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG40840.10959477573189533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA57350.035.83888632
GCGGCAC59300.034.79519333
TCGCCGG57100.034.47518548
CCCGTCG57150.034.17877644
CGGCACC61550.033.54142434
CCGTCGC58800.033.2953445
ACCCGCG64350.032.3077525
CCCCCGA65300.031.70777519
CCGCGCG68450.030.55613127
CCCCGAC68100.030.46656820
CCCGCGC70100.029.81836526
GCACCCC70700.029.57251236
CACCCCC71300.029.09076937
TAGTCCG4100.027.6965666
GGCACCC74800.027.61378935
CGTCGCC71800.027.38208246
GCGCGGG74050.027.1297462
AGTCCGC78650.026.8199277
TACGGGT8400.026.6274686
AGGAGGG109800.026.61050870