FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726455
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN101060.2711960831406793No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC79830.21422504766594525No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC60910.16345293314960196No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG46030.12352222152152649No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA42780.11480079593071699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG11000.056.6103634
TCTACGG11200.056.213825
TACGGCC11550.053.321047
CTACGGC12250.050.5529446
GCGCGGT39550.047.6724559
TGGCGCG41100.046.28816615
ACGGCCA14650.042.272948
CGGCATA3200.041.047631
CTACTCG46550.040.44829637
TACTCGG47750.039.52979338
CGGTATG2550.039.3108371
GAACGCG15600.039.15395724
CGCGGTG49350.038.3450210
CCCGATC16150.037.74901631
AGGATCG50050.036.95192359
CGGGTAG6000.036.8708531
GTAGTCC51700.036.8396527
GCCGGTT7500.036.6957173
CGTGCCT51700.036.65484220
ATCTCGT16400.036.6153435
Produced by FastQC (version 0.11.5)