FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3720300
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG192910.5185334516033653No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN100070.26898368411149637No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG56700.15240706394645595No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG49380.1327312313523103No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA45440.12214068757895868No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG39770.10689998118431308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA52900.039.3884432
GCGGCAC55100.037.89153333
CCCGTCG52800.036.6028844
TCGCCGG53500.036.00256348
CGGCACC58000.035.8944234
ACCCGCG58850.035.34415425
CCGTCGC55150.034.991445
CCCCCGA59600.034.9317319
CCCCGAC61800.033.8079620
CCGCGCG63100.032.98858627
GCACCCC64450.032.5467136
CACCCCC66450.031.5294337
CCCGCGC67100.031.01156226
GGCACCC69850.030.11854635
CGTCGCC65900.029.40898346
AGTCCGC72450.028.846157
CAGTCCG70950.028.3398786
ACCCCCC74950.027.9248238
TAGTCCG3100.027.7661636
GCGCGGG70400.027.70711162