FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3720300
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN99410.26720963363169636No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC78210.2102249818563019No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC60670.16307824637798027No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG47320.12719404349111632No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA42130.11324355562723437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG12400.056.071934
TCTACGG13050.053.279085
TACGGCC13000.053.2185067
GCGCGGT39950.049.799059
CTACGGC13900.049.7720386
TGGCGCG43100.045.99972515
CGGCATA3050.044.298271
ACGGCCA16400.041.9755258
GAACGCG15750.041.6509724
TACTCGG48400.040.57225438
CTACTCG47900.040.54110337
CGTGCCT50650.039.40198520
CCCGATC16400.039.28922731
CGCGGTG50900.039.2887610
CGGCCAT17700.039.28159
ATCTCGT16000.039.2602335
CGGTATG2850.038.897991
GCCATAC17450.038.85803211
TGAACGC17200.038.52242723
CCATACC17550.038.44049512
Produced by FastQC (version 0.11.5)