FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3629052
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG154710.42630968087533605No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC91930.2533168441785899No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC75870.2090628626980269No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG70570.19445849770132806No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG59560.16411999607611022No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT53820.14830319323062882No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45190.12452287815109842No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT44580.12284199840619534No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA43280.119259795671156No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG40770.11234338885196464No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT37990.10468298608011128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT19900.047.89274670
CGCGGCA43900.037.86055432
GCGGCAC46600.035.67976833
ATGGCCG21550.035.0993669
TCGCCGG45950.035.07349848
CCGTCGC45650.034.71240645
CCCGTCG46350.034.2408844
CGGCACC48700.034.08000634
GCACCCC54050.031.1770336
CCCCCGA53750.031.0686119
ACCCGCG55700.030.58697525
GTTCCGT4550.030.3518581
CACCCCC56400.029.76783437
GTGCCGT6050.029.6745871
GACGTAT3050.029.66902544
GATAGGC29300.028.79863440
CGTCGCC56550.028.65502246
GGCACCC58650.028.42474635
CCCCGAC59300.028.10886220
CGCCCTA7950.027.51360361