FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3629052
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG193350.532783768322967No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC179280.49401331256757963No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC156110.4301674376669169No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA126470.34849321530801985No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT79040.21779792629039207No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG54650.15059029189992318No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45130.12435754571717351No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC38990.10743852664552617No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG37780.10410432256137417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG39650.063.6341444
TCTACGG39700.063.3803525
TACGGCC39600.063.0303277
CTACGGC40850.061.5151026
ACGGCCA42250.059.6481328
CGGCCAT43800.057.2224549
GCCATAC44750.056.39209711
CCATACC46650.054.53871512
CCGAACT13400.054.52477367
GCGCGGT82200.054.2711039
GGGTCTA33700.054.0033042
CATACCA48050.052.94819613
GGCCATA47700.052.8329910
GCCGGTT13700.052.3310053
GCGTCTA9200.052.076682
CCTGGTT69550.052.00077470
CGCGGTG87650.051.01457210
TGGCGCG87850.050.7302615
CGAACTT14950.050.4753368
ATACCAC51300.049.5862514