FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3143902
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG211320.6721583560810738No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC121430.38623977464946424No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC103340.3286998131621151No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN75740.24091081719468355No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA70610.22459351468334574No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT59310.18865091850827412No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG58040.18461135238948287No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG38860.12360436171356486No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC36200.11514353818916746No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC32170.1023250724736331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG41050.062.256154
TCTACGG41550.061.5069855
TACGGCC41300.061.4625937
CTACGGC42950.059.342576
ACGGCCA43600.058.29988
CGGCCAT45100.056.3616879
GCCATAC45000.055.88058511
GCGTCTA8900.055.057992
GGGTCTA33800.054.5431182
CGTCTAC10000.053.7912523
CCATACC46800.053.73132712
CATACCA47350.053.25191513
GGCCATA48300.052.77111410
GCGCGGT60200.052.2399569
CGGTTCA9150.051.667165
CGGGTAG3600.051.5961531
TACCACC49800.050.90726515
CCGAACT10000.050.18682567
GCCGGTT9550.049.1506463
ATACCAC52100.049.0544614