FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497019
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG135780.38827355527665136No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG65990.18870357867658138No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC64670.18492893518736958No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC59130.16908687084628365No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG53050.15170063416870197No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG39980.1143259444687032No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG36750.10508950623373794No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC36580.10460337790558186No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT35470.10142924588056285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT18250.043.06039470
CGCGGCA45250.033.93001632
GCGGCAC46450.032.9873733
TCGCCGG45550.032.19804448
CGGCACC47800.031.9900834
CCGTCGC45800.031.8283945
CCCGTCG46500.031.4834744
AGGACCG6650.031.25873670
ATGGCCG20100.031.06884469
GTTCCGT3500.030.645751
GCACCCC53750.028.72297536
GACGTAT5250.028.48148544
CACCCCC55300.027.9275337
CCCCCGA55700.027.80601119
CGCGAAT500.00767339227.646763
ACCCGCG57050.027.11381725
TTATCGG2050.026.972457
TCGGATA5450.026.72128934
CGCCCTA11250.026.58403861
GTGCCGT5600.026.5678881