FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497019
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG185640.5308521343464248No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC112540.32181695323931614No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC99160.28355579423503274No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA96740.2766356145048111No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG56330.16108005132371314No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC45200.12925294372149537No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT44920.1284522617692383No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC38900.11123759979571173No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG36010.10297341821705859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG38500.063.5433544
TCTACGG38550.063.3713045
TACGGCC39050.062.648387
CTACGGC40000.061.0740936
ACGGCCA42750.057.3070038
CGGCCAT43150.056.775779
GCCATAC43750.055.99232511
GGGTCTA31600.055.4457442
CCATACC46200.052.94824612
CATACCA47050.052.06512513
GCGTCTA9100.052.0267222
GGCCATA47450.051.7711110
GCCGGTT9300.051.645143
CCTGGTT67050.051.5030870
CCGAACT9700.049.8374667
GCGCGGT55250.049.2198269
CTGAACG49450.049.02483722
CGTCTAC10900.048.8193323
TACCACC50250.048.4779715
ATACCAC50900.048.39851814