FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3585477
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG186180.5192614539153367No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC114390.3190370486270028No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC100220.2795165050563705No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA98620.2750540583582045No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG55660.1552373645124484No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT45320.12639880272555087No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC45040.12561787455337184No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC37900.10570420616280622No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG36420.10157644296700272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC39200.062.3884777
GTCTACG39500.062.0774084
TCTACGG39800.061.6962665
CTACGGC40550.060.3898666
ACGGCCA43300.056.640578
CGGCCAT44150.055.237149
GCCATAC44750.054.72733711
GGGTCTA33500.054.228982
CCATACC46050.053.10736512
CATACCA47000.052.25221313
CCTGGTT67450.051.7389670
CGTCTAC10100.051.6323473
GCGCGGT55500.051.534089
GCGTCTA8900.051.2241132
CCGAACT10150.050.4985967
GGCCATA48900.050.36604710
TGGCGCG58700.048.71452715
TACCACC50300.048.6793215
ATACCAC50800.048.33818414
CTGAACG50950.048.30005622