FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3545701
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG138710.3912061394911754No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG66460.1874382526896656No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC64150.18092332094556196No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC58970.16631408006484472No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG51430.14504889160140688No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG39560.11157173151374017No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC36730.10359023504802013No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT36670.10342101604167978No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG36440.10277234318404176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT17550.045.91631370
TACGCGA350.001338130439.4887352
CGCGGCA41950.032.87221532
GTCCGTT3700.031.751082
GCGGCAC44200.031.28432733
ATGGCCG20400.030.80007769
TCGCCGG42350.030.6091248
CCCGTCG43050.030.5384144
CGGCACC45200.030.52070634
CCGTCGC42650.030.43005245
GTGCCGT5750.028.8989941
CGTACGT1452.1827873E-1128.60578519
GTTCCGT4750.028.4236811
GCACCCC48900.028.3675936
GACGTAT4550.028.28252444
CGGATAG4450.028.04887235
AGGACCG7400.027.3952570
CCCCCGA51150.027.1657119
TCGGATA4600.027.12655434
CACCCCC52650.026.5552437