FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3408940
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG127100.3728431711910447No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC70070.20554776558108973No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG59280.17389569778288852No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG57260.16797010214318822No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC49500.1452064278045375No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC41080.12050666776182625No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA40140.11774921236513403No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG34890.10234853062828914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA39250.034.91436832
GCGGCAC40350.033.96954733
TGGCCGT16050.033.7639170
TCGCCGG39300.032.2533248
CGGCACC42700.031.86044934
CCCGTCG40400.031.42654244
CCGTCGC40450.031.13706245
GTTCCGT3550.029.5106121
CCCCCGA46650.029.39716719
ACCCGCG47400.028.73308825
GCACCCC48200.028.23613536
AACGACG500.00745238727.81279442
CACCCCC50450.027.32737237
ATGGCCG15250.027.27193569
CCCCGAC50400.027.21307420
GATAGGC22550.027.11884940
GGCACCC51300.026.6573735
ATAGCGC6150.025.52880770
GTCCGTT2600.025.301892
GTGCCGT6050.024.8198071