FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418289
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG129410.37858121416884294No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC73060.2137326598189913No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG61390.17959277287555264No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG57530.16830057376658322No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC48740.1425859545521166No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC42360.12392164618029664No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA39970.11692984414132332No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG35340.10338505609092737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA38350.037.37486632
CCGTCGC37700.035.5338945
GCGGCAC40400.035.396133
CGGCACC40300.035.31685634
TCGCCGG37700.035.1998948
TGGCCGT14300.035.17520570
CCCGTCG38300.034.87612544
CCCCCGA43450.033.17212719
ACCCGCG45200.031.82512925
GCACCCC45800.031.46579736
GTGCCGT5100.030.680741
CACCCCC47700.030.07257537
CCCCGAC48750.029.78024120
CGGATAG3650.029.49037735
CGTATCA3550.029.41245346
GACGTAT3550.029.39602744
GGCACCC49200.029.28832235
ATGGCCG13350.029.00208769
CCGATCG16700.028.61407970
ACGTATC3700.028.2125945