FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418289
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG113440.3318619344356197No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC104440.3055329727825822No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA93940.27481585085403837No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC91040.26633207432139294No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC48060.1405966552272204No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT38060.11134225339051204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG23600.060.4629255
GTCTACG23600.060.316174
TACGGCC24050.059.330737
CTACGGC24650.057.8848846
CGGCCAT27800.051.825819
GCGCGGT48100.050.6183329
GCCATAC28400.050.6089511
ACGGCCA28400.050.608958
GCGTCTA5300.050.3182032
CCATACC29950.047.87415312
GGGTCTA22750.047.8033752
TGGCGCG50750.047.63259515
CATACCA30650.046.6677813
CCGAACT7800.045.77113367
CGGCATA2750.045.625651
GAACGCG31350.045.42674624
CGAACTT8200.045.1635168
CTGAACG31650.045.09507822
TGAACGC31750.045.06809623
CCTGGTT45550.044.71253670