FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3417723
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG128690.3765372442412682No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC71640.20961324250092828No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG58600.1714591849602791No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG56450.16516844694552485No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC49750.14556475173675573No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC42630.124732168171616No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA40820.11943624454059033No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG34540.10106143768819183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT12800.038.33257770
CGCGGCA38900.038.16757632
GCGGCAC39900.037.3023133
CCCGTCG38550.036.9838244
TCGCCGG38650.036.94079248
CCGTCGC39550.036.2330745
CGGCACC41200.036.13059234
GTTCCGT3550.034.4826971
GCACCCC46000.032.5991736
CCCCCGA46200.032.3938919
ACCCGCG46750.032.1028725
CACCCCC47250.031.59724637
GGCACCC48500.030.91381335
AGGACCG5250.030.83166170
CCCCGAC51350.029.14674620
ATGGCCG13200.028.50671269
AGTCCGC53700.027.7969517
TCCGCCC58850.027.0122269
CAGTCCG52350.026.9260046
CGTACGT1554.9112714E-1126.82074719