FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3417723
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG110230.3225246750541223No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC98880.2893154301855358No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA91420.2674880322366675No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC87000.25455544524819596No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC45290.13251512776196317No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT38360.11223846988184824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG23350.061.843064
TACGGCC23700.061.076787
TCTACGG24250.059.548735
CTACGGC24800.058.0884556
GCCATAC27400.052.70279711
CGGCCAT27950.051.9135139
ACGGCCA28000.051.6971328
GCGCGGT48800.049.886579
CCATACC29550.049.2169312
CCGAACT7800.047.57039667
CATACCA30500.047.1162813
GGGTCTA23100.046.9416772
CCTGGTT44250.046.90318770
GCGTCTA5750.046.3926472
GAACGCG31250.046.23824724
TGGCGCG52800.045.9078415
TGAACGC31550.045.90437723
CTGAACG31650.045.86544822
CGCGGTG53650.045.50588610
GGCCATA32400.045.1040610
Produced by FastQC (version 0.11.5)