FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4050396
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG158910.39233200901837745No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC128750.317870153930628No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG105240.25982644659929544No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC95410.235557214652592No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG74660.18432765586377234No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT66590.16440367806012054No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT58630.1447512786404095No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA51440.12699992790828354No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT49730.12277811848520491No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG47290.1167540161505196No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG46260.11421105491907459No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT45740.11292722983135477No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG44450.10974235605604983No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG44160.10902637668020608No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA43890.10835977519235156No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG42620.10522427930503586No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG42010.10371825372136453No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC41900.1034466753374238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT27900.050.3084970
ATGGCCG29800.037.2860169
CGCGGCA52300.036.10090332
GCGGCAC53250.035.6630733
CGGCACC54200.034.66208634
GTTCCGT4650.033.4800341
CCGTCGC52950.033.1242245
TCGCCGG53550.032.60926448
CCCGTCG53950.032.4968944
GTACCGT751.634744E-632.2896351
CCCCCGA60450.031.72346319
GCACCCC59750.031.52154236
GGCACCC60600.031.06746135
ACCCGCG62150.030.4406825
CACCCCC63200.029.97439837
GTGCCGT6700.029.9488071
GATAGGC38550.029.46381840
TATGGCC35500.029.11767268
CGTACGT2150.028.92796719
TAGTCCG3600.028.7830146
Produced by FastQC (version 0.11.5)