FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491056
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG136300.39042627789413864No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG59850.17143809781338368No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC53400.15296231283600148No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG50100.14350958563827107No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC44120.12638009817086865No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA38680.1107974206085494No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG37210.10658666031137856No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC36740.10524036280139877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT14400.042.0487570
CGCGGCA43750.035.4323932
GCGGCAC45200.034.2244133
CGGCACC45900.033.63221734
CCCGTCG43200.033.08428244
TCGCCGG42450.032.90077248
CCGTCGC43750.032.28069745
ATGGCCG15500.031.59404869
CCCCCGA51350.030.47648819
GCACCCC51650.030.10080336
CACCCCC52650.029.73366237
ACCCGCG51800.029.69403625
AGGACCG6300.028.03250170
CCCCGAC55650.027.93896920
GGCACCC57400.027.0804235
GACGTAT3400.026.5924344
AGTCCGC58500.026.5057267
CAGTCCG56900.026.2778026
GTGCCGT5300.025.5617081
TCCGCCC63400.025.4398299