FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491056
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG137670.39435059191259036No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC115990.33224903868628863No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC101750.29145908859668823No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA95160.2725822788290993No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT53880.1543372549738532No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG39390.11283118918745502No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC38280.10965163549367299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG28800.062.2442635
GTCTACG28800.062.0039374
TACGGCC29200.061.0369267
CTACGGC29600.060.795816
ACGGCCA32400.055.222218
GCCATAC33250.053.90609711
CGGCCAT33500.053.305649
CCATACC34350.051.98434412
GCGCGGT59950.051.8965879
GCGTCTA6050.049.763082
GGCCATA35900.049.7420310
GGGTCTA25750.049.724212
TGGCGCG63000.049.16087715
CATACCA36350.048.83852413
CCGAACT8650.048.6903967
CGCGGTG64950.047.90146610
CGGGTAG5700.047.5941241
GAACGCG38450.046.56585324
CCTGGTT53300.046.5524270
TGAACGC38650.046.3202423