FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3465879
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG135470.39086765579525423No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG58280.16815359105150526No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC52220.15066884908561437No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG50360.14530224511588546No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC43540.12562469722687952No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC38690.11163113311226387No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA37770.10897668383691411No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG36810.10620682372350565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT14600.039.33444270
CGCGGCA43150.035.28329532
GCGGCAC45500.033.39392533
CCCGTCG42950.033.36540244
GTCCGTT2500.033.2260132
TCGCCGG43550.032.8749648
CCGTCGC43950.032.7754545
GTTCCGT3100.032.660331
CGGCACC46750.032.6555934
CCCCCGA52100.029.36833219
GCACCCC52850.029.09855736
CACCCCC53200.029.04561437
ACCCGCG52400.028.94847525
ATGGCCG15650.028.71969
GGCACCC55750.027.32832135
CGCCCTA10300.026.57265561
CCCCGAC58000.026.32345820
GTTATCG1759.094947E-1225.709496
AGTCCGC60500.025.5133617
CGCTTCC7550.025.4331441