FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3479055
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG136260.3916580795647094No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG59410.1707647622702142No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC54240.1559044050755162No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG48870.14046917913053975No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC43090.12385547224749249No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC39780.1143413944303841No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA39550.1136802953675639No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG37210.10695433098930601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT15100.043.2256670
CGCGGCA41600.034.76962732
GCGGCAC43900.033.03651433
CCCGTCG42050.032.59565744
CCGTCGC41650.032.57836545
TCGCCGG41700.032.40709748
ATGGCCG16000.032.3191269
CGGCACC45500.031.72685234
GTTCCGT3900.031.17721
CCCCCGA50250.029.07466319
ACCCGCG50650.028.99885625
GCACCCC51050.028.62573636
TCGCCGT3800.028.3407781
CACCCCC52300.027.8144437
CGTACGT1800.026.92736419
CCCCGAC55800.026.30843220
GGCACCC56600.025.75412835
GTCCGTT2700.025.641052
AGTCCGC57250.025.5735367
CAGTCCG55000.025.486976