FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3482284
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG137610.39517167468247855No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG58320.16747628855084767No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC54990.15791359923544432No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG48950.140568661257956No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC43160.12394164289874117No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC38990.11196674366593878No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA38560.11073192192250833No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG36630.10518958246943672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT16250.042.40717770
CGCGGCA41050.037.4126732
CCGTCGC41000.035.72719645
CCCGTCG41300.035.5416544
GCGGCAC43200.035.4781133
TCGCCGG41150.035.46681248
CGGCACC44100.034.995534
CCCCCGA48100.032.0829719
ACCCGCG48050.031.9191325
CACCCCC49150.031.21377637
GCACCCC49600.031.1333436
ATGGCCG17550.030.6508169
GACGTAT2450.029.8145644
CCCCGAC52750.029.38852120
GGCACCC54450.028.34795635
TCGCCGT3350.028.1284621
AGTCCGC57200.027.1521387
CAGTCCG56150.026.6742256
GTTCCGT4100.026.3879031
GTCCGCC59100.026.3377558