FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527533
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG271130.7686108110115484No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC128120.363200004082173No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC114310.3240508309915173No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG75890.21513618724474018No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA70650.20028161324075494No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT69980.19838226885474922No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG52120.14775198417704383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG55500.063.784895
GTCTACG55350.063.7712824
TACGGCC56250.063.11887
CTACGGC56850.062.210586
ACGGCCA60350.059.0562138
CGGCCAT61400.057.8782049
GGGTCTA44700.057.279942
GCCATAC62350.056.77563511
CCATACC63850.055.38716512
GCGTCTA11500.055.0626532
CGTCTAC13200.054.7319343
CATACCA64750.054.51027313
CCTGGTT92150.054.5078270
GGCCATA65550.054.42383610
CGGTCTA6850.053.7564242
GCGCGGT60800.053.470149
TACCACC66600.053.04775215
TGGCGCG64800.050.2742715
CTGAACG69600.050.1784722
ATACCAC70800.049.85226414