FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3533454
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG128420.3634404183555241No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC77430.219134025800251No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC62780.17767317757638842No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG61990.17543740487353168No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG40190.11374139864280108No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC37600.10641146028786563No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36450.10315685445459316No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG36310.1027606415705426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT24900.052.90574370
ATGGCCG28200.037.40046769
CGCGGCA37500.036.9589232
GCGGCAC39550.034.9692633
TCGCCGG39800.033.19776548
CGGCACC42150.033.15117334
CCCGTCG40650.032.49659344
CCGTCGC40650.032.42823845
AGGACCG7700.031.95358570
TAGTCCG3350.030.8288066
GTCGGTT2500.030.2944414
CCCCCGA45400.029.8215919
GCACCCC47450.029.54389836
TATGGCC33650.028.89741368
GTGCCGT5650.028.7499471
GATAGGC25550.028.64185540
GTTCGTA1700.028.3512153
GTATGGC33850.027.91643767
GTTATCG2100.027.8682616
CACCCCC50800.027.80994237