FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437022
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG131950.383907929597192No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG60680.17654818619141804No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC59310.1725621773733191No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC49360.14361269727106782No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG47750.13892840953592966No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG36840.10718581376552143No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC36240.10544011647292337No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA36040.10485821737539068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT15250.044.49442370
CGCGGCA42850.035.76079632
GCGGCAC43400.035.47617333
CGGCACC45250.033.80375734
CCCGTCG41650.033.32228544
CGTACGT1154.5474735E-1133.0902419
TCGCCGG42150.032.8969848
CCGTCGC42650.032.47892845
ATGGCCG16750.031.56909669
GCACCCC50900.030.12753536
CCCCCGA51350.029.77740919
GACGTAT3450.029.23854444
ACCCGCG52350.028.96113425
CACCCCC52650.028.9356437
GGCACCC55200.027.77983335
AGTCCGC57100.026.772967
CAGTCCG55600.026.2511236
TCGCCGT3200.026.0200231
GTACGTA1607.6397555E-1125.94840220
GTCCGCC60500.025.5542058
Produced by FastQC (version 0.11.5)