FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424203
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG146200.42696066792769No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG65740.19198628118718428No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC56480.1649434919600269No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG48560.14181402212427244No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC47980.14012019731306818No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG39380.115004863905557No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC37840.11050746699304918No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA37330.10901806931423166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT15050.038.7239570
GCGGCAC45800.033.9256633
TCGCCGG42950.033.92332548
CGCGGCA46000.033.6963432
CCGTCGC43700.033.37761745
CCCGTCG44300.033.065244
CGGCACC47100.032.9233734
ATGGCCG14500.030.81991669
AGGACCG5300.030.33418370
GCACCCC51700.030.2094936
ACCCGCG53100.029.27636525
CACCCCC54200.028.69388237
CCCCCGA55650.028.21992919
TCGCCGT2700.028.1973511
GGCACCC55800.027.98117835
CGTTGCG1306.1136234E-926.6198751
GTACGGG9650.026.1559435
AGTCCGC61050.026.0519757
CCCCGAC61150.025.91294520
CAGTCCG60100.025.8313266