FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424203
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG138190.40356836320743833No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC112950.32985778004399857No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC91890.2683544170716514No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA78580.22948405804211958No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT50950.14879374850147611No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC43820.12797138487408602No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG38240.111675622035259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG28800.062.0511254
TACGGCC29000.061.5039867
TCTACGG29000.061.3847925
CTACGGC29950.059.6685266
ACGGCCA31600.056.5529178
GCCATAC32650.054.41422711
CGGCCAT32900.054.213249
GCGTCTA5900.053.325652
CCATACC33750.052.435912
GCGCGGT52350.052.4270489
GGGTCTA26500.051.4040222
GGCCATA35900.049.5844310
CCTGGTT50300.049.424470
CGGTCTA3650.049.2557872
GAACGCG35700.049.24563224
CATACCA36050.048.99459513
TGGCGCG55900.048.91209415
CTGAACG35950.048.60015522
TGAACGC36400.048.00775523
CGTCTAC7300.047.824463