FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480262
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG134670.3869536259051761No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC111150.3193725070124031No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC90400.2597505590096378No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA80160.23032748683863455No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT49420.14200080338779092No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC42590.12237584411748312No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG39010.11208926224519879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG26300.063.2250984
TCTACGG26450.062.8692555
TACGGCC26600.062.2547877
CTACGGC27800.059.5675356
ACGGCCA29500.056.017638
GGGTCTA23100.053.8754232
GCCATAC30950.053.0581211
CGGCCAT31300.052.7961779
GCGCGGT53450.052.7790229
CATACCA32250.051.23946413
CCATACC32600.050.58330512
GAACGCG32900.049.75182724
TGGCGCG57250.049.5770115
CCGAACT7500.048.21182667
GGCCATA34350.048.10830710
CCTGGTT48650.048.0097770
TGAACGC34350.047.94907823
CGCGGTG58800.047.85926410
CTGAACG34500.047.8353922
ATCTCGT34750.047.10732335