FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3440016
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG143320.41662596918153866No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66790.19415607369268048No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG50150.14578420565485742No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG37910.11020297580011257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA43650.040.0086832
GCGGCAC46400.037.8039933
CCCGTCG44150.037.0124644
TCGCCGG44150.036.85820448
CCGTCGC45250.036.1281445
CGGCACC48550.035.92934434
ACCCGCG50850.034.46085725
CCCCCGA53850.032.8001419
GCACCCC54450.032.06625436
CCCCGAC56500.031.02518520
CACCCCC56300.030.84253137
CCGCGCG57400.030.66726927
CGTACGT1401.4551915E-1129.56954819
CGTCGCC55550.029.38084646
GGCACCC59700.029.35015935
AGTCCGC61450.028.9351487
CCCGCGC61100.028.74600426
CCCCCGT56800.028.43418742
CAGTCCG60300.028.1726366
GTCCGCC64250.027.8886878
Produced by FastQC (version 0.11.5)