FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3423514
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC80760.23589796916267902No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN64530.18849053925294304No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC62290.1819475544718088No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG58260.1701760238164646No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT37200.10866028297240789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG12950.058.019474
TCTACGG13450.055.606375
TACGGCC13700.054.334517
CTACGGC14500.051.5796976
GCGCGGT39900.048.1945089
TGGCGCG40700.046.99386215
ACGGCCA16900.045.2691658
GCCATAC16400.045.178411
CCGAACT6550.043.96615667
CGCGGTG45750.042.33323710
CGGCATA2950.042.3149071
CGGCCAT18350.041.8798459
GAACGCG17800.041.5304724
CTACTCG48850.039.73686237
ATCTCGT18100.039.48206335
TACTCGG49300.039.3199438
CGTGCCT49600.039.0907520
GGGTCTA15550.039.0225372
CGGTTCA7600.039.0006565
CGGGTAG5250.038.9677771
Produced by FastQC (version 0.11.5)