FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431463
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG141930.4136136685722679No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66300.19321204978751044No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG48830.14230082037894623No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG37620.10963253865770957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA41300.037.59739732
GCGGCAC42950.036.00642833
TCGCCGG41550.035.19954748
TGGCCGT12000.034.70441470
CCCGTCG42250.034.55965444
CCGTCGC42800.034.21059845
CGGCACC45550.034.11143534
CCCCCGA48050.032.6772819
ACCCGCG48150.032.02289225
GCACCCC50300.031.12354736
ATGGCCG10350.030.4959869
CCCCGAC51200.030.40703820
CACCCCC53400.029.52868837
GACGTAT2800.028.6254244
CGTCGCC51300.028.48473446
CCGCGCG54600.028.44491627
GGCACCC55250.028.07512535
GTCGCCG53850.027.27909947
AGTCCGC57950.027.2519347
CCCCCGT54300.026.99160642
Produced by FastQC (version 0.11.5)