FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3428001
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC74740.2180279410653614No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65200.19019831091064443No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC61130.1783255022387683No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG57650.16817381325151304No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT35660.10402564059928804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG14700.058.372664
TACGGCC14900.058.2957657
TCTACGG14900.057.6026085
CTACGGC16100.053.0951546
GCGCGGT39900.049.9325839
TGGCGCG42800.046.9451115
ACGGCCA19100.046.1986548
TGAACGC19250.045.39042323
CCGAACT6050.045.2293467
GCCATAC19850.044.10646411
CGGCCAT20150.043.9629829
GAACGCG19600.043.8859924
ATCTCGT19300.043.6740635
CCATACC20400.043.25019512
CATACCA20700.043.12287513
CCCGATC20400.042.0974231
CGCGGTG47750.042.08470510
GCCCGAT20500.041.5395830
CTGAACG20950.041.19936422
CGTCTGA20800.040.92305439
Produced by FastQC (version 0.11.5)