FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658163
Sequences flagged as poor quality0
Sequence length35-260
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA253581.5292827062236944No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT243261.4670451578041483No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA226931.3685626805084905No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT210731.2708642033382724No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT194211.1712358797054332No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGTCTGGTCCTACGCTGGTGAA190831.1508518764439926No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT184201.1108678700465515No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG168071.013591546790032No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT167881.0124457004528506No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC164460.9918204663835823No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG156280.9424887661828178No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT151920.9161946081295989No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT149350.9006955287266691No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT145150.875366293904761No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT141770.8549822906433203No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA136630.8239841318374611No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT128610.7756173548680075No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA116730.703971804943181No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA112820.6803914934780235No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA112480.6783410316114881No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG112380.6777379545919189No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA110780.6680887222788109No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG110070.6638068754398694No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC109420.6598868748126692No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC103220.6224960995993759No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC100930.6086856358512402No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG100640.6069367124944893No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA97730.5893871712250244No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG97610.5886634788015412No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT96650.5828739394136764No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC92080.5553133196193618No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG88880.536014854993146No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC84150.5074893119675207No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA83590.5041120806579329No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC83500.5035693113403206No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC82640.49838284897202506No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC78650.4743200758912121No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCGACGACCACGTTCCCA77730.46877176731117504No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC76910.46382653575070726No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA76130.4591225349980671No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC76100.45894161189219634No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA74230.44766407162625144No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG74120.4470006869047253No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC73840.44531207124993144No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC72650.4381354547170574No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCGGGCTGTTATCCTTTG70840.427219760662854No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG66510.40110652571550565No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC66180.39911637155092716No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGTCTGGTCCTACGCTGGTGAA55070.3321145146767839No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC51630.3113686652036018No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC50490.3044935871805124No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA50450.3042523563726847No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC49920.3010560481689677No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC46490.2803705063977425No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG44700.2695754277474531No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC44610.2690326584298407No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC40260.2427988080785785No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC39300.2370092686907138No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAA39130.23598403775744606No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36780.22181172779756875No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC33460.20178957074786977No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCAGTAGTCCTTGACCAG32220.19431141570521113No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC24950.15046771638252693No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC24200.14594463873575758No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGTCTGGTCCTACGCTGGTGAA21930.1322547903915357No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG21430.12923940529368946No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA19720.11892678825905535No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGTCTGGTCCTACGCTGGTGAA19690.11874586515318458No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA18680.11265478725553518No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCTCGTATCCGACGGGGA17500.1055384784246181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT753150.0236.23771
TGTCCAG739500.0228.91861
GTCCAGC751200.0225.385452
AGCAGTG788900.0225.009492
TCCAGCA753600.0224.860843
AGCACGC752200.0224.795556
CACGCTT755350.0223.97058
ACGCTTC755500.0223.845759
CCAGCAC762200.0222.164554
CAGCACG761750.0221.929535
GTGGTAT803150.0213.797496
GCACGCT791900.0213.541287
TGGTATC817800.0209.58197
GTATCAA837100.0204.416479
GCAGTGG873800.0203.036093
GGTATCA858300.0199.635948
AGTGGTA864850.0199.021685
CAGTGGT889350.0193.879994
AAGCAGG24550.0182.332581
AAGGCAG47650.0148.42091
Produced by FastQC (version 0.11.5)