FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446851
Sequences flagged as poor quality0
Sequence length35-260
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT196751.3598497702942458No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT170601.1791124310658112No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT155141.072259686726553No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT153591.061546766045709No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA146951.0156539961613187No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG124370.8595909323074733No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG121400.8390635939706299No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA119170.8236508113136736No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA118530.8192274118067444No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT113030.7812138222940718No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT110460.7634511086490593No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT105640.7301373811124988No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT101070.6985515440083326No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT99740.6893591669079954No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA98500.68078883036332No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA97620.6747066560412924No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA88670.6128481785615796No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA88510.6117423286848473No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA88180.6094615133140869No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA86280.5963295460278909No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC85400.5902473717058633No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC85220.5890032905945394No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG84040.5808476477536388No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT82670.5713788081841185No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA78380.5417282083642337No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCGACGACCACGTTCCCA75830.5241037259538127No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA72840.503438156382378No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG72120.49846183193708266No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCGGGCTGTTATCCTTTG71850.4965957102700969No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG69040.47717422180998603No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC66850.4620379016222127No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG65300.45132498094136847No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC65140.4502191310646362No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC60900.42091410933123036No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG59030.40798948889692166No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC58690.4056395579088655No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC57410.39679275889500715No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT55410.38296963543585344No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC55360.3826240573493746No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC54470.3764727674100512No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA54370.3757816112370935No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC51980.35926297870340484No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC51930.358917400616926No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC50210.3470295144420538No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC49860.3446104678367019No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC49310.34080910888543464No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC46030.31813918641242256No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG45710.315927486658958No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC45200.3124025901768738No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC43520.30079116647118465No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG40870.282475527887806No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC39900.2757713130101164No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG35310.24404724467135872No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC32830.2269065715820081No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC30140.20831447052944638No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCAGTAGTCCTTGACCAG28940.20002059645395412No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC28410.1963574687372784No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG28210.19497515639136304No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC26180.18094468608032202No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGTCTGGTCCTACGCTGGTGAA24630.1702317653994779No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC23860.1649098628677037No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC21680.14984265829722618No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCTCGTATCCGACGGGGA20260.14002824064122704No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGTCTGGTCCTACGCTGGTGAA17850.12337137687294683No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA17250.11922443983520073No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAA15680.10837328791976507No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGTCTGGTCCTACGCTGGTGAA15530.10733655366032854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT572600.0229.251531
TGTCCAG753100.0222.620041
AGCAGTG596650.0219.873522
GTCCAGC767300.0218.408662
AGCACGC770350.0217.528736
TCCAGCA771450.0217.339893
ACGCTTC772900.0216.538579
CACGCTT773950.0216.426218
CAGCACG777100.0215.51885
CCAGCAC777950.0215.343494
GTGGTAT593400.0210.687296
GCACGCT800650.0209.091937
TGGTATC607050.0205.622187
GCAGTGG651400.0201.195633
GTATCAA625000.0199.267469
AGTGGTA635250.0197.249335
GGTATCA642200.0194.14918
CAGTGGT653500.0192.277924
AAGCAGG20200.0180.706571
GTGGGTA32200.0148.969966
Produced by FastQC (version 0.11.5)