FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005319588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42800794
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA15723063.6735440001416797No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA9500092.219606019458424No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG8834302.064050494016536No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG7555001.7651541698034856No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT6569231.5348383490268895No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT6162101.4397162818988825No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT3932140.9187072557579189No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA3534310.8257580455166322No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG3134650.7323812731137651No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA2517380.5881619859668958No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT2508410.5860662304535752No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG2206350.5154927733349993No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA1981560.4629727196182388No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC1965160.4591410150007965No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT1682400.393076820023479No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG1638690.3828643926558932No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA1464440.342152531095568No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA1116660.2608970291532442No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA1113750.26021713522417367No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA1030620.24079459834319897No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCT1021340.23862641426698766No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT1001240.23393023970536622No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTT998160.23321062688696848No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG948580.22162672963496893No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA870220.20331865806040889No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT790730.18474657269208605No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC771040.18014619074590066No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGAT746210.1743448964988827No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGAC741170.17316734825059554No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT676130.15797136847508017No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT665900.15558122589968776No Hit
CAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA665890.15557888949443321No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG542060.12664718322748872No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG539030.1259392524353637No Hit
AGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGA538140.12573131236770982No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG532200.12434348764651423No Hit
GCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT498920.11656793095941163No Hit
CCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGG483120.11287641065724154No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACT469100.10960077049037922No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT469040.10958675205885199No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA466980.10910545257641716No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT458530.10713119013633253No Hit
CACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC444140.10376910297505229No Hit
GGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT440820.10299341643054566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)