FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24754673
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT15850.011.9094811
CTACCGT17500.09.1167748
GTATCGA14850.08.77687554
CGTATGG16000.08.70741212
CGCGATT2902.2268196E-48.5238831
TTCGCGC4752.7275746E-88.5152967
TACCGTA19000.08.3968249
CGTATCG15300.08.3718583
CCGCAAT14250.08.2003291
ACTACCG19600.08.13997657
CTCGACT19500.08.0668921
CTCGGAT28000.07.9454771
CGACCTA6908.367351E-117.81628754
CCGTATG18300.07.73582911
GTCCGAT9350.07.69097041
CCCGGAT27950.07.6380871
CTCCGAT23300.07.61928031
TCGGATT18750.07.55053422
ACCGTAT19350.07.548187710
CCGTATC20000.07.5280632
Produced by FastQC (version 0.11.5)