FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31130702
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT8250.011.1662771
CCCGTAT19150.010.7943741
GTATCGA15350.010.2461784
CGTATCG16450.09.6976393
CCCGGAT40600.08.9100121
CGTATGG17800.08.70921312
TACCGTA21050.08.3250769
CCCGGTT26150.08.2485571
CTCGGTT26200.08.2328171
CGGTAAT14550.08.18448451
ACCGTAT20500.08.1100510
CTACCGT23900.07.8024968
CCGTATG19600.07.794746411
CTTGGAT114400.07.77763651
ATCGAAG38950.07.7294876
TCGTACG10100.07.58777629
CGGGGTT51600.07.5331191
CTGGAAT161200.07.5267451
CTCGGGT34450.07.50044631
CGCGTAT3000.00291785327.4895761
Produced by FastQC (version 0.11.5)