FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28158628
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG40600.018.3835443
CGTATGC41050.018.33589744
CTCGTAT43000.016.99040642
TCACGAT49800.014.9337635
TCTCGTA50100.014.672641
ACGATCT52400.013.9855437
CACGATC54250.013.9158636
ATCTCGT56000.013.32806740
ATCACGA59950.012.18059534
CATCACG62500.011.97626533
GATCTCG64650.011.64944339
CGACCGT8700.011.6225121
GATCGGA56600.011.2350951
GTATGCC68800.011.07244345
CGATCTC70500.010.52286338
ACACGTC71050.010.40450913
CTCGGAT32800.010.0704811
ACGTCTG73400.010.05181315
CCCGTAT17950.010.0145691
ATCGGAA62950.09.9588472
Produced by FastQC (version 0.11.5)