FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27549665
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG12150.012.05804929
TATCTCG27900.09.9366741
CCCGTAT13750.09.806081
TCGTATG29150.09.63682745
TACCGTA16600.08.9340439
CGACCGT7050.08.9251561
CTACCGT18500.08.8668568
CTCGGAT33400.08.8139721
CTCGTAT31450.08.78813444
CGTACGG18650.08.33892730
TAACGCG3003.1094678E-48.264400525
TCGGATT20250.08.1009812
GTACGGA20800.08.01006131
CGTATGG17600.07.915934612
CCCGGAT35150.07.79976221
TCTCGTA38800.07.60991143
ACCGTAT19200.07.607231610
CTCGAAT30250.07.5031361
CGGTAAT12500.07.37090351
CTCGTTT26450.07.3066721
Produced by FastQC (version 0.11.5)