FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24944134
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA10350.09.98580744
CCCGTAT15050.09.8539151
CGCGTAT2352.584987E-49.5616661
CTCGGAT28300.09.1308841
CGTATCG10700.08.8198243
GTATCGA11500.08.7924164
CTCGCAT27800.08.7293191
TCGCATT19900.08.4684252
CCCGGAT31150.08.4397431
CTCGGTT21750.08.2647951
CTCAGAT102650.08.2305771
GACGCTA13450.08.1859174
CGCGTAA3603.109155E-58.1141361
GTCCGAT10250.08.1110911
ACGCTAC15050.07.91248465
CGACGGT12550.07.905154741
CTTGGAT96650.07.8115791
ATTGCGA22000.07.761929513
CGCCAAT16750.07.64647771
CGACGAC21200.07.63129141
Produced by FastQC (version 0.11.5)