FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28859238
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT18750.012.4648671
CGTATCG14250.012.4583863
TCGTACG13200.010.92979929
GTATCGA16250.010.0952964
CCGTATC23700.09.1027472
GACGCTA14850.08.77710154
TACCGTA15650.08.7586669
CGTACGG18100.08.2199830
CCCGGAT37700.08.0472591
ATCGAAG37500.08.0297926
CCGCAAT18000.07.99029971
CTCGGAT35650.07.75356051
CTCGTTT32100.07.7009311
CGTATGG14700.07.643317712
GTTCGAT20500.07.56398771
CTACCGT18450.07.55136258
ACTACCG20400.07.4904777
CTCGCAT29850.07.37797741
CGCGAAC4303.5393103E-57.31669861
ACCGTAT16000.07.30310210
Produced by FastQC (version 0.11.5)