FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26996825
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT13500.012.1509921
CGCGTAT2606.003176E-610.3712381
CGTATCG12400.09.60440353
CTCGCGT7850.09.4464131
CGTATGG14150.09.368793512
CCGTATG15250.08.54567211
CTCGGAT35850.08.5245691
CGGGGAT31650.08.306821
TACCGTA17650.08.2747099
CGTATGC28050.08.1712781
CCCGGTT22400.08.0253621
CCCGGAT31400.08.0151391
CTCGCAT27300.07.65496061
CGGGGTT42100.07.63267231
CTTGGAT94900.07.62451651
CTACCGT20350.07.61865578
CTCGGTT22000.07.5584321
TCGTACG8351.8189894E-127.557826529
TATGCCG27550.07.5038213
ACCGTAT18600.07.489710
Produced by FastQC (version 0.11.5)