FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27337364
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA26900.020.21451836
CGTAATC27500.019.84384738
ACGTAAT28650.019.12603237
CGTACGT28750.018.75761834
TCGTATG30200.018.38035445
GTACGTA30250.017.97588535
ACCGTAC33050.016.61087632
CTCGTAT35050.016.0911144
CCGTACG36200.015.2760833
CCCGTAT17800.013.6347181
AATCTCG42600.013.28640141
TCTCGTA43250.013.1388643
ATCTCGT48150.011.70811542
CACCGTA47700.011.6590931
GTATCGA16950.011.268774
CGTATGG16750.011.0002912
TACCGTA18000.010.7355999
ACCGTAT19900.010.04933410
GTAATCT58850.09.92422439
CGTATCG17800.09.8469913
Produced by FastQC (version 0.11.5)