FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28266228
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT20550.013.0127981
GTATCGA15850.010.6331194
CGTATCG15500.010.4382933
TACCGTA16800.08.9611929
CCGTATC25950.08.7460652
CGCGTAT3155.801738E-58.5606411
GTTCGAT19250.08.4049931
CTACCGT20950.08.3660568
CGTATGG16950.08.08655412
ATCGAAG39300.08.061896
CCCGGAT40600.07.9149031
CCCGATT17700.07.87143661
CGCTAGT6002.757588E-97.8650891
CGCGAAT5554.5994966E-87.69300841
CCGTATG17550.07.682055511
CGAGAAT27950.07.55756041
CCGGAAT25900.07.37487651
ACCGTAT18000.07.365132310
CGCAAAT18550.07.2684691
CTCGGAT35500.07.21625851
Produced by FastQC (version 0.11.5)