FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31610515
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG13600.011.93320429
CCCGTAT18150.09.6563621
TACCGTA17100.09.460119
CCCGGAT42350.09.0727351
CGTACGG18800.08.87230830
ACCGTAT17600.08.68075410
CTCGGAT38800.08.5129711
CGTATGG15950.08.45195712
CGCGTAA2400.00331796338.4261041
CTACCGT20050.08.2925258
CGTATGC33250.08.17623844
CGACCGT8150.07.99532131
TATCTCG34700.07.99233239
CGTATCG13300.07.9403513
CTCGCAT28500.07.8052331
CGTCGAT11550.07.78168341
CTCGCGT10200.07.71016071
GTACGGA23050.07.61900231
TCGTATG35850.07.610164643
CTCGTTT34450.07.5659641
Produced by FastQC (version 0.11.5)