FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25611501
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT17200.013.9779991
CGCGTAA5550.09.7164571
GTATCGA14600.09.5416494
GTCGCTA25600.09.50959627
ATCGAAG50950.09.12834556
TCGTATG21350.08.87173543
CGTATCG24200.08.8204933
CTCGGAT31150.08.8001791
CTCGACT24450.08.2709111
AAGCGAC31100.08.24459620
CGCTATG31350.08.12492229
CCGACAT38750.08.1179431
CGTATGC24650.08.11195444
CGGTCTA22200.08.09635344
CTCGTAT24100.08.04652942
TCGAAGG54250.08.0346687
CCGGAAT22550.07.97137361
CCCGGAT35700.07.9303441
TATCGAA22950.07.92991075
CGACCGT5453.3411197E-87.83333641
Produced by FastQC (version 0.11.5)