FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29928901
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT19950.014.6418881
GTATCGA15950.013.3829414
CGTATCG17500.012.9679693
TCGTATG30900.010.94542243
CGCGTAA3103.6929123E-810.872421
CGTATGC31300.010.69576244
CTCGTAT33150.010.13450342
TACCGTA23150.09.9966869
CGTATGG19100.09.8814712
CTACCGT23100.09.7266098
TCGTACG10850.09.55584529
CCGTATC26950.09.4212462
ACCGTAT20100.09.38975910
ATCGAAG40850.09.1304086
CCGTATG21700.08.59392811
TCTCGTA39950.08.52236141
ACTACCG27500.08.497187
CTCGGAT36300.08.4183881
TCGAAGG41650.08.3076817
AATCTCG44150.08.27340139
Produced by FastQC (version 0.11.5)